中国血吸虫病防治杂志 ›› 2021, Vol. 33 ›› Issue (2): 138-.

• 论著 • 上一篇    下一篇

免疫学技术检测吡喹酮治疗后血清抗日本血吸虫抗体转归的meta分析

王丽萍,邓王平,贾铁武,秦志强,许静*   

  1. 中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心)、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室、世界卫生组织热带病合作中心、国家级热带病国际联合研究中心(上海200025)
  • 出版日期:2021-04-30 发布日期:2021-04-30
  • 作者简介:王丽萍,女,硕士研究生。研究方向:血吸虫病流行病学
  • 基金资助:
    国家科技重大专项(2018ZX10101002?002);国家自然科学基金(82073619)

Conversion of serum anti-Schistosoma japonicum antibodies detected by immunological tests following treatment with praziquantel: a meta-analysis

WANG Li-Ping, DENG Wang-Ping, JIA Tie-Wu, QIN Zhi-Qiang, XU Jing*   

  1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research); NHC Key Laboratory of Parasite and Vector Biology; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Shanghai 200025, China
  • Online:2021-04-30 Published:2021-04-30

摘要: 目的 评价免疫学技术检测日本血吸虫感染病人和病畜经吡喹酮治疗后血清抗血吸虫抗体转归情况。方法  检索中国知网、万方数据、PubMed、ScienceDirect等数据库,筛选1991—2020年公开发表的关于日本血吸虫病免疫学检测的文献,提取纳入研究文献相关数据,使用RevMan 5.3统计软件绘制漏斗图分析发表偏倚,采用meta分析进行综合评价。结果 最终纳入40篇文献,其中中文文献33篇、英文文献7篇,均为采用间接血凝试验(IHA)或酶联免疫吸附试验(ELISA)开展的研究。效应量合并分析结果显示,吡喹酮治疗后 6个月内,ELISA和IHA法检测血清抗日本血吸虫抗体阴性率分别为45.36%[95%可信区间(CI):(43.96%,46.76%)]和20.83%[95% CI:(19.69%,21.97%)];治疗后6 ~ 12个月,ELISA和IHA法检测抗体阴性率分别为62.95%[95% CI:(61.59%,64.31%)]和55.61%[95% CI:(54.21%,57.01%)];治疗12个月后,ELISA和IHA法检测抗体阴性率分别为85.92%[95% CI:(84.94%,86.90%)]和86.90%[95% CI(85.95%,87.85%)]。结论 随着吡喹酮治疗后时间推移,IHA、ELISA法检测血清抗日本血吸虫抗体阴性率升高。在治疗后12个月内,2种方法检测血清抗日本血吸虫抗体阴性率总体较低,但在治疗12个月后如无新的疫水接触史,抗体阴性率较高。

关键词: 日本血吸虫病, 吡喹酮, 抗体转归, 免疫学检测, Meta分析

Abstract: Objective To evaluate the conversion of serum antibodies against Schistosoma japonicum in humans and livestock detected by immunological tests following treatment with praziquantel. Methods The studies pertaining to serological tests of schistosomiasis japonica published from 1991 to 2020 were retrieved in electronic databases, including Chinese National Knowledge Infrastructure, WanFang Data, PubMed and ScienceDirect. Data were extracted from included studies. The publication bias was assessed with funnel plots using the software RevMan version 5.3, and the conversion of antibodies against S. japonicum was evaluated through meta?analysis. Results A total of 40 publications were included in the final meta?analysis, consisting of 33 Chinese publications and 7 English publications, and all immunological tests were performed with indirect hemagglutination test (IHA) and enzyme?linked immunosorbent assay (ELISA). Pooled analysis showed that the negative rates of serum anti?S. japonicum antibody were 45.36% [95% confidential interval (CI): (43.96%, 46.76%)] and 20.83% [95% CI: (19.69%, 21.97%)] detected by ELISA and IHA within 6 months post praziquantel treatment, 62.95% [95% CI: (61.59%, 64.31%)] and 55.61% [95% CI: (54.21%, 57.01%)] within 6 to 12 months after treatment and 85.92% [95% CI: (84.94%, 86.90%)] and 86.90% [95% CI: (85.95%, 87.85%)] over 12 months after treatment, respectively. Conclusions The negative rate of the serum anti?S. japonicum antibody by IHA and ELISA increased with the time of post?treatment with praziquantel. The overall negative rates of anti?S. japonicum antibody detected by IHA and ELISA are low within 12 months post praziquantel treatment. However, a high negative rate of anti?S. japonicum antibody is detected if there is no new contact with infested water after 12 months of praziquantel treatment.

Key words: Schistosomiasis japonica, Praziquantel, Antibody conversion, Immunological test, Meta?analysis

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