中国血吸虫病防治杂志 ›› 2019, Vol. 31 ›› Issue (5): 504-.

• 论著 • 上一篇    下一篇

旋毛虫成虫排泄?分泌蛋白对卵清蛋白诱导的小鼠变应性鼻炎的保护作用

袁圆1, 2,王黎源1, 2,梅娟3,程洋4,王伟5,褚亮3,唐洁2,李楠1,李徽徽1,贺文欣1,杨浪浪2,陈杰2,白永生2,吴玥2,梁金宝2,孙思颖2,张祥2,杨小迪1, 2*   

  1. 1蚌埠医学院基础医学院(蚌埠 233000);2安徽省感染与免疫重点实验室;3蚌埠医学院第二附属医院;4江南大学无锡医学院病原感染与免疫组;5蚌埠医学院第一附属医院
  • 出版日期:2019-11-04 发布日期:2019-11-05
  • 作者简介:袁圆,女,硕士,讲师。研究方向:寄生虫与免疫
  • 基金资助:
    国家自然科学基金(81441120);安徽高校科研创新平台团队项目(2016-40);安徽省学术和技术带头人及后备人选科研活动经费资助项目(2018H174);安徽省高等学校自然科学研究重点项目(KJ2018A0227);蚌埠医学院自然科学研究项目(BYKY1757);蚌埠医学院自然科学研究重点项目(BYKY1624ZD);蚌埠医学院科技发展基金项目(BYKF1853)

Protective effect of excretory-secretory protein from adult Trichinella spiralis on ovalbumin-induced allergic rhinitis in mice

YUAN Yuan1, 2, WANG Li-Yuan1, 2, MEI Juan3, CHENG Yang4, WANG Wei5, CHU Liang3, TANG Jie2, LI Nan1, LI Hui-Hui1, HE Wen-Xin1, YANG Lang-Lang2, CHEN Jie2, BAI Yong-Sheng2, WU Yue2, LIANG Jin-Bao2, SUN Si-Ying2, ZHANG Xiang2, YANG Xiao-Di1, 2*   

  1. 1 Department of Basic Medical Sciences, Bengbu Medical College, Bengbu 233000, China; 2 Anhui Provincial Key Laboratory of Infection and Immunity, China; 3 The Second Affiliated Hospital of Bengbu Medical College, China; 4 Department of Pathogenic Infection and Immunity, Wuxi Medical School, Jiangnan University, China; 5 The First Affiliated Hospital of Bengbu Medical College, China
  • Online:2019-11-04 Published:2019-11-05

摘要: 目的 探讨旋毛虫成虫排泄?分泌蛋白(Adult?worm excretory?secretory protein, AES)对卵清蛋白(Ovalbumin, OVA)诱导的小鼠变应性鼻炎的保护作用。方法 18只雌性BALB/c小鼠随机分为3组:A组为空白对照组,B组为变应性鼻炎组,C组为AES治疗组。A组小鼠给予PBS;B组小鼠经腹腔注射抗原佐剂混悬液进行基础致敏,隔日1次,共7次,后以5% OVA溶液滴鼻进行局部激发,每日1次,共7次,诱导建立变应性鼻炎模型;C组小鼠在诱导变应性鼻炎基础上,于基础致敏和局部激发时分别加入25 μg AES进行干预。末次激发后观察各组小鼠30 min,并进行行为学评分;采用酶联免疫吸附试验检测小鼠血清中IFN?γ、IL?4、IL?5、IL?10及TGF?β水平变化,HE染色后镜下观察小鼠鼻黏膜病理改变。结果 3组小鼠行为学评分差异有统计学意义(F = 110.12, P < 0.01);B组小鼠行为学评分显著高于A组[(7.17 ± 0.75)分和(1.33 ± 0.52)分,P < 0.01],且鼻黏膜病理损伤更为显著;经AES治疗后,C组小鼠行为学评分[(3.83 ± 0.75)分]显著下降(P < 0.01),鼻黏膜病理损伤亦显著缓解。3组小鼠血清中IFN?γ水平差异有统计学意义(F = 7.50,P < 0.01),B组小鼠血清中IFN?γ水平显著低于A组(P < 0.05);经AES治疗后,C组小鼠血清中IFN?γ水平较B组显著升高(P < 0.05)。3组小鼠血清中IL?4 (F = 470.81,P < 0.01)、IL?5水平(F = 68.20,P < 0.01)差异均有统计学意义,B组小鼠血清中IL?4、IL?5水平均显著高于A组(P均 < 0.01);经AES治疗后,C组小鼠血清中IL?4、IL?5水平均较B组显著降低(P均 < 0.01)。3组小鼠血清中IL?10(F = 174.91,P < 0.01)、TGF?β水平(F = 9.39,P < 0.01)差异均有统计学意义;经AES治疗后,C组小鼠血清中IL?10、TGF?β水平均显著高于B组(P均 < 0.05)。结论 旋毛虫AES对OVA诱导的小鼠变应性鼻炎有显著的保护作用。

关键词: 旋毛虫, 排泄?分泌蛋白, 变应性鼻炎, 卵清蛋白, 保护作用, 小鼠

Abstract: Objective To investigate the protective effect of excretory?secretory protein (AES) from adult Trichinella spiralis on ovalbumin (OVA)?induced allergic rhinitis in mice.Methods Eighteen female BALB/c mice were randomly divided into three groups, including the blank control group (Group A), OVA?induced rhinitis group (Group B) and AES treatment group (Group C). Mice in Group A were given PBS. Mice in Group B were intraperitoneally injected with antigen adjuvant suspension for systemic sensitization, once every other day for seven times; then, local excitation was intranasally induced with 5% OVA solution once a day for seven times to establish a mouse model of allergic rhinitis. In addition to induction of allergic rhinitis, mice in Group C were given 25 μg AES at baseline sensitization and local excitation. Following the final challenge, mice were observed for 30 min in each group, and the behavioral score was evaluated. The serum levels of IFN?γ, IL?4, IL?5, IL?10 and TGF?β were determined using an enzyme?linked immunosorbent assay in mice, and the pathological changes of mouse nasal mucosa were observed under a microscope. Results There was a significant difference in the mouse behavioral scores among the three groups (F = 110.12, P < 0.01). The mouse behavioral score was significantly higher in Group B than in Group A (7.17 ± 0.75 vs. 1.33 ± 0.52, P < 0.01), and more remarkable pathological damages of mouse nasal mucosa were seen in Group B than in Group A, while the mouse behavioral score was significantly decreased in Group C than in Group B (P < 0.01), and the pathological damages of mouse nasal mucosa remarkably alleviated in Group C relative to Group B. There was a significant difference in serum IFN?γ level among the three groups (F = 7.50, P < 0.01) and the serum IFN?γ level in Group B was significantly lower than in group A and C (both P < 0.05). There were significant differences in serum IL?4 (F = 470.81, P < 0.01) and IL?5 levels (F = 68.20, P < 0.01) among the three groups, and significantly greater serum IL?4 and IL?5 levels were detected in Group B than in Group A (P < 0.01), while significantly lower serum IL?4 and IL?5 levels were detected in Group C than in Group B (P < 0.01). There were significant differences in serum IL?10 (F = 174.91, P < 0.01) and TGF?β levels (F = 9.39, P < 0.01) among the three groups, and significantly greater serum IL?10 and TGF?β levels were seen in Group C than in Group B (both P < 0.05). Conclusion T. spiralis AES has a remarkable protective activity against OVA?induced allergic rhinitis in mice.

Key words: Trichinella spiralis, Excretory?secretory proteins, Allergic rhinitis, Ovalbumin, Protective effect, Mouse

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