中国血吸虫病防治杂志 ›› 2019, Vol. 31 ›› Issue (5): 474-.

• 论著 • 上一篇    下一篇

安徽及周边省份绵羊和山羊隐孢子虫分子特性分析

李文超,汪凯,唐莉,陈苗,李慧,阚珍珍,顾有方*   

  1. 安徽科技学院动物科学学院(凤阳 233100)
  • 出版日期:2019-11-04 发布日期:2019-11-05
  • 作者简介:李文超,男,博士,副教授。研究方向:人兽共患原虫分子流行病学
  • 基金资助:
    安徽省牛羊产业技术体系项目;安徽省高校优秀青年人才重点项目(gxyqZD2016220);安徽科技学院校级重点项目(ZRC2016478);安徽科技学院重点学科项目(AKZDXK2015A04)

Molecular characterization of Cryptosporidium species in sheep and goats in Anhui Province and neighboring provinces

LI Wen-Chao, WANG Kai, TANG Li, CHEN Miao, LI Hui, KAN Zhen-Zhen, GU You-Fang*   

  1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China
  • Online:2019-11-04 Published:2019-11-05

摘要: 目的 调查安徽及周边省份绵羊和山羊隐孢子虫流行情况及分子特性。方法 选择安徽省及其周边的河南、江苏和山东部分地区的7个规模化绵羊场和10个规模化山羊场,分别采集832份和781份新鲜绵羊和山羊粪便样品,利用隐孢子虫SSU rDNA基因特异的巢氏PCR技术对所有样品进行检测,调查上述地区绵羊和山羊隐孢子虫感染和虫种分布;对获得的微小隐孢子虫和泛在隐孢子虫进行gp60基因扩增与分析,以鉴定其基因亚型。结果 安徽及周边省份绵羊和山羊隐孢子虫感染率分别为5.8%(48/832)和8.7%(68/781)。SSU rDNA基因分析显示,绵羊感染的隐孢子虫为肖氏隐孢子虫和泛在隐孢子虫,山羊感染的隐孢子虫为微小隐孢子虫。gp60基因分析显示,泛在隐孢子虫基因亚型均为XIIa亚型2,微小隐孢子虫基因亚型均为IIdA19G1。结论 人兽共患泛在隐孢子虫XIIa亚型2和微小隐孢子虫 IIdA19G1基因亚型的鉴定,提示绵羊和山羊可能为人隐孢子虫感染的潜在来源。

关键词: 隐孢子虫, 绵羊, 山羊, 基因分型, 分子特性

Abstract: Objective To investigate the prevalence and molecular features of Cryptosporidium in sheep and goats from Anhui Province and neighboring provinces. Methods A total 832 and 781 fresh fecal samples were collected from seven large?scale sheep farms and ten large?scale goat farms in Anhui Province and neighboring provinces of Henan, Jiangsu and Shandong. The prevalence and species of Cryptosporidium were investigated in the fecal samples from the sheep and goats in the study areas using nested PCR assay based on the Cryptosporidium?specific SSU rDNA gene, and the subgenotypes of C. parvum and C. ubiquitum were characterized by amplification and sequencing of the 60 kDa glycoprotein (gp60) gene. Results The overall prevalence of Cryptosporidium was 5.8% (48/832) in sheep and 8.7% (68/781) in goats in Anhui Province and neighboring provinces, respectively. The SSU rDNA gene?based PCR assay identified C. xiaoi and C. ubiquitum in sheep and C. parvum in goats, and subtyping revealed that all C. ubiquitum subgenotypes belonged to XIIa subtype 2 and C. parvum subgenotypes belonged to IIdA19G1. Conclusion The identification of zoonotic C. ubiquitum XIIa subtype 2 and C. parvum subtype IIdA19G1 suggests that sheep and goats may serve as a potential source for human Cryptosporidium infections.

Key words: Cryptosporidium, Sheep, Goat, Genotyping, Molecular feature

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