中国血吸虫病防治杂志 ›› 2018, Vol. 30 ›› Issue (5): 504-507.

• 论著 • 上一篇    下一篇

不同地理来源恶性疟原虫微卫星标记位点多态性研究 

燕贺|丰俊|尹建海|陈田木|周水森*   

  1. 中国疾病预防控制中心寄生虫病预防控制所、世界卫生组织热带病合作中心、科技部国家级热带病国际联合研究中心、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室(上海 200025)
  • 出版日期:2018-12-01 发布日期:2018-12-01
  • 通讯作者: 周水森
  • 作者简介:燕贺|女|硕士|助理研究员。研究方向:疟疾分子流行病学、疟原虫抗药性
  • 基金资助:
    国家自然科学基金青年基金(81602904);上海市卫生和计划生育委员会科研课题青年项目(20164Y0216)

Polymorphism analysis of microsatellite marker loci of Plasmodium falciparum from different geographical origins

YAN He| FENG Jun| YIN Jian-hai| CHEN Tian-mu| ZHOU Shui-sen*   

  1. National Institute of Parasitic Diseases| Chinese Center for Disease Control and Prevention; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases| Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology| National Health Commission| Shanghai 200025| China
  • Online:2018-12-01 Published:2018-12-01
  • Contact: ZHOU Shui?sen

摘要: 目的 比较东南亚与非洲输入性恶性疟原虫在Polyα、TAA87两个微卫星位点多态性的差异。方法 收集缅甸(92份)和加纳(126份)输入性恶性疟病例血液样本共218份,对所收集样本的Polyα、TAA87微卫星位点进行片段扩增,毛细管电泳检测扩增产物长度,应用Excel 2010和GenALEx 6.0软件计算等位基因频率和期望杂合度。结果 共获得146例单一感染样本的有效数据。Polyα、TAA87两个微卫星位点分别检测到16个和12个等位基因型,2个微卫星位点的平均期望杂合度为0.86 ± 0.02。缅甸输入样本在Polyα及TAA87位点的等位基因数分别为10个和8个,期望杂合度分别为0.86和0.81;加纳输入样本在Polyα及TAA87位点的等位基因数分别为15个和11个,期望杂合度分别为0.91和0.86 。此外,长度为174 bp(Polyα)和113 bp(TAA87)的等位基因仅在东南亚缅甸样本中测出,其等位基因频率占17%以上。结论 东南亚与非洲输入性恶性疟原虫在Polyα、TAA87两个微卫星位点具有不同等位基因类型和数量,建议将这两个位点作为区分不同地理来源疟原虫的候选基因。 

关键词: 恶性疟原虫;微卫星;多态性;东南亚;非洲

Abstract: Objective To compare the genetic diversity of imported Plasmodium falciparum by Polyα and TAA87 microsatellite markers in Southeast Asian and African geographical isolates. Methods Ninety?two and 126 filter paper samples from patients infected with P. falciparum from Southeast Asia (Myanmar) and Africa (Ghana) were collected, respectively. Two neutral microsatellite loci, Polyα and TAA87 were amplified by PCR. The length of PCR fragments was detected by capillary electrophoresis. The allele frequency and expected heterozygosity (He) were calculated by Excel 2010 and GenALEx 6.0 software. Results A total of 146 P. falciparum samples were analyzed as single infection samples with a total of 26 alleles in locus Polyα and 12 alleles in locus TAA87. The mean He value of the two loci was 0.86 ± 0.02. Ten alleles in locus Polyα and 8 alleles in locus TAA87 were distributed in Myanmar isolates, with the He values of 0.86 and 0.81 respectively. Fifteen alleles in locus Polyα and 11 in locus TAA87 were detected in Ghana isolates, with the He values of 0.91 and 0.86 respectively. In addition, the haplotype of 174 bp (Polyα) and 113 bp (TAA87) were only detected in Myanmar isolates with more than 17% gene frequency, whereas they were absent in Ghana isolates. Conclusions The two different geographical sources of imported P. falciparum strains have different allele frequencies and haplotypes at the two neutral microsatellite markers, Polyα and TAA87. Therefore, these two microsatellite loci may be considered as the potential molecular marker candidates for identifying P. falciparum strains with different geographical sources.

Key words: Plasmodium falciparum; Microsatellite; Polymorphism; Southeast Asia; Africa

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