中国血吸虫病防治杂志 ›› 2018, Vol. 30 ›› Issue (4): 455-459.

• 防治管理 • 上一篇    下一篇

疟疾生物样本库的构建与应用

唐建霞|徐岁|刘耀宝|曹园园|张梅花|顾亚萍|张超|周华云|朱国鼎|曹俊*   

  1. 国家卫生健康委员会寄生虫病预防与控制技术重点实验室、江苏省寄生虫与媒介控制技术重点实验室、江苏省寄生虫病防治研究所、世界卫生组织消除疟疾研究与培训合作中心(无锡214064)
  • 出版日期:2018-10-08 发布日期:2018-10-09
  • 通讯作者: 曹俊
  • 作者简介:唐建霞|女|博士| 副研究员。研究方向:媒介生物学
  • 基金资助:
    国家重点研发计划(2016YFC1200500);江苏省自然科学基金(BK20150001);江苏省重点研发计划(BE2016631);江苏省“科教强卫工程”项目

Construction and application of malaria biobank

TANG Jian-xia| XU Sui| LIU Yao-bao| CAO Yuan-yuan| ZHANG Mei-hua| GU Ya-ping| ZHANG Chao| ZHOU Hua-yun| ZHU Guo-ding| CAO Jun*   

  1. Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention| Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology| Jiangsu Institute of Parasitic Diseases| WHO Collaborating Center for Research and Training on Malaria Elimination| Wuxi 214064| China
  • Online:2018-10-08 Published:2018-10-09
  • Contact: CAO Jun

摘要: 目的 探索建立具有流行病学资料的疟疾生物样本库,实现疟疾样本管理信息化,为消除疟疾提供保障,并为相关研究提供资源。方法 以江苏省寄生虫病防治研究所为依托,自2011年起持续收集江苏省内疟疾病例(包括本地和输入性疟疾病例)资料,按照标准化操作流程收集入选患者的末梢血涂片、滤纸血、静脉血样本及分离培养虫株,并进行处理、分装和存储。应用样本库管理软件录入病例流行病学资料,并进行样本入库,定期对样本进行质量控制。结果 建立了疟疾生物样本库,实现从样本入库、保存,到出库的全程信息化管理。2011-2017年样本库共收集了江苏省报告疟疾病例中99.42%(2 223/2 236)的血涂片、92.58%(2 070/2 236)的滤纸血,94.50%(2 113/2 236)的静脉全血及2.06%(46/2 236)的恶性疟原虫培养株。依托样本库,对全省99.42%(2 223/2 236)的疟疾病例样本进行了镜检复核及82.74%(1 850/2 236)的病例样本进行了核酸检测复核。此外,还利用样本库开展了疟原虫抗药性监测、疟疾分子流行病学及疟疾诊断技术等相关研究。结论 疟疾生物样本库的建立不仅为消除疟疾工作提供了保障,同时也为疟疾相关研究提供了资源。 

关键词: 疟疾;生物样本库;信息化

Abstract: Objective To establish a systemized malaria biobank with well?rounded epidemiologic data and a computer?aid management system, thus to provide qualified sources for malaria elimination and human malaria research. Methods The malaria biobank was based on the platform of Jiangsu Institute of Parasitic Diseases. The blood smear samples, dried blood samples, whole blood samples and parasite strains isolated from patients (from both local and imported cases) were collected since 2011 according to a standardized operational procedure. The biobank management software was applied to input of the epidemiological data and samples, and the quality of samples was monitored regularly. Results A standard malaria biobank was established. The Information Management System was applied to input, storage and output of samples. Totally 99.42% (2 223/2 236) of the blood smear samples, 92.58% (2 070/2 236)of the dried blood samples, 94.50% (2 113/2 236) of whole blood, and 2.06%(46/2 236) of the isolated stains in 2 236 reported cases were included in the malaria biobank in Jiangsu Province from 2011 to 2017. Based on the malaria biobank, 99.42%(2 223/2 236) of the blood smears and 82.74% (1 850/2 236)of DNA (from dried blood and whole blood samples) from malaria patients in Jiangsu Province were re?checked. Moreover, the samples in the malaria biobank were used in the studies of the mechanism of parasite drug resistance, malaria molecular epidemiology, and diagnosis technology development and evaluation. Conclusion The establishment of malaria biobank provides a guarantee for malaria elimination in Jiangsu Province and also provides a qualified resource for malaria research.

Key words: Malaria; Biobank; Informationization

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