关键词: 血吸虫病；噁二唑?2?氧化物；杂环化合物；超高效液相色谱?四级杆飞行时间质谱法

Abstract: Objective To develop an ultra?performance liquid chromatography/quadrupole?time of flight mass spectrometry （UPLC?QTOF?MS） method for the determination of an oxadiazole?2?oxide heterocyclic compound F?2015?14. Methods Mouse plasma and liver homogenate specimens were extracted with ethyl acetate and chromatographed on a Waters CORTECS column （C18， 1.6 μm， 2.1 mm × 150 mm） by using a mobile phase of 10% acetonitrile?0.1% formic acid with by a volume fractionation by gradient elution. Then， UPLC?QTOF?MS was performed to determine F?2015?14 in mouse plasma and liver homogenate specimens. Results The linearity of F?2015?14 in plasma ranged from 12.5 to 250 mg/mL with a correlation coefficient of 0.990 and a detection limit of 8.8 mg/mL. F?2015?14 in liver homogenates ranged from 12.5 to 250 mg/mL. The linearity was good with a correlation coefficient of 0.992 and a limit of detection of 5.6 mg/mL. If the concentration of plasma and liver homogenate specimens was 12.5 mg/mL， the accuracy and the matrix effect were 80% to 120%， and the inter?day and intra?day precision was within 20%. If the concentrations of plasma and liver homogenate specimens were 100 mg/mL and 200 mg/mL， the accuracy and the matrix effect were 85% to 115%， and the inter?day and intra?day precision was within 15%. Conclusion The UPLC?QTOF?MS established in this study has a high sensitivity and good reproducibility for the determination of F?2015?14， which provides bases for the development of novel anti?schistosomiasis drugs.

Key words: Schistosomiasis； Oxadiazole?2?oxide； Heterocyclic compound； Ultra?performance liquid chromatography/quadrupole?time of flight mass spectrometry