关键词: 等温扩增技术；分子生物学；分子诊断；寄生虫

Abstract: The in vitro nucleic acid amplification technique based on polymerase chain reaction （PCR） has been successfully applied to scientific researches. In recent years， the emergence of isothermal amplification technology is increasingly applied in the molecular diagnosis and disease detection because of its advantages of constant temperature， high efficiency， short time?consuming， and less reliance on equipment and instruments. The principle， characteristics and application of the partial isothermal amplification technique in the pathogen detection in parasitic and other diseases are reviewed in this paper， and the prospects of the wide development of the technique are also discussed.

Key words: Isothermal amplification technology； Molecular biology； Molecular diagnosis； Parasite