中国血吸虫病防治杂志 ›› 2018, Vol. 30 ›› Issue (2): 189-193,207.

• 论著 • 上一篇    下一篇

3种常见人体寄生虫NAD1基因的电子克隆及对比分析

谢健1|赵永霞2|杨明理1|张志敏1|贾启1*   

  1. 1 遵义医学院医学遗传学教研室(遵义 563003);2 茅台学院酿酒工程系
  • 出版日期:2018-05-11 发布日期:2018-05-14
  • 通讯作者: 贾启
  • 作者简介:谢健|男|副教授。研究方向:分子生物学与功能基因组学
  • 基金资助:
    贵州省科学技术基金(黔科合LH字[2014])

In silico cloning and comparative analysis of NAD1 gene in three common human parasites

XIE Jian1| ZHAO Yong-xia2| YANG Ming-li1| ZHANG Zhi-min1| JIA Qi1*   

  1. 1 Department of Medical Genetics| Zunyi University| Zunyi 563003| China; 2 Department of Brewing Engineering| Moutai University| China
  • Online:2018-05-11 Published:2018-05-14
  • Contact: JIA Qi

摘要: 目的 利用电子克隆法对人体寄生虫NAD1基因进行电子克隆和生物信息学分析,为NAD1基因的进一步深入研究奠定基础。方法 分别选取华支睾吸虫、蛔虫和日本血吸虫的一个表达序列标签(EST)片段作为种子序列,以NCBI中的EST数据库作为目标参考序列,用电子克隆的方法分别克隆3种寄生虫的NAD1基因,并对其编码蛋白的理化性质、氨基酸组成、亚细胞定位、二级和三级结构进行对比分析。结果 3种人体寄生虫NAD1蛋白理化性质、亚细胞定位、相对分子量、等电点差异较小,蛔虫和日本血吸虫的NAD1蛋白在三级结构上高度相似。进化分析显示,3种人体寄生虫的NAD1蛋白分属不同分支,存在一定的遗传距离。结论 通过电子克隆得到的3个NAD1基因是属于不同种属的同一基因,可广泛应用于研究寄生虫的种间和种内遗传变异。

关键词: 寄生虫;电子克隆;NAD1基因;生物信息学分析

Abstract: Objective To in silico clone the NAD1 gene of three common parasites and analyze their bioinformatics, so as to lay the foundation for further research on the NAD gene. Methods By using the in silico cloning method, the full length cDNA(s) of NAD 1 genes of Clonorchis sinensis, Ascaris lumbricoides and Schistosoma japonicum were got, then their physical and chemical properties, compositions of amino acids, subcellular localizations, binary and ternary structures were contrastively analyzed. Results The three kinds of NAD1 proteins were similar in the relative molecular weight, subcellular localization, and physical and chemical properties. The NAD1 proteins were highly similar in binary and ternary structures of A. lumbricoides and S. japonicum. The phylogenetic analysis showed that C. sinensis, A. lumbricoides and S. japonicum belonged to the different evolutionary branches with a certain of genetic distance. Conclusion The three NAD1 genes got from C. sinensis, A. lumbricoides and S. japonicum by in silico cloning belong to the same gene of different species, which can be widely used in the researches of heritable variation of parasites.

Key words: Parasite; In silico cloning; NAD1 gene; Bioinformatics analysis

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