关键词: 日本血吸虫；TIGIT；Th1/Th2反应平衡；流式细胞术

Abstract: Objective To investigate the role of TIGIT signal in Th1/Th2 balance in the process of Schistosoma japonicum infection. Methods Male C57BL/6 mice were infected with cercariae of S. japonicum， and normal uninfected mice served as the controls. The percentages of TIGIT+ cells， Ki67+CD3+CD4+TIGIT+ cells， Ki67+CD3+CD4+TIGIT? cells， IFN?γ+CD3+CD4+TIGIT+cells， IFN?γ+CD3+CD4+TIGIT? cells， IL?4+CD3+CD4+TIGIT+ cells and IL?4+CD3+CD4+TIGIT? cells were evaluated in mouse spleen by flow cytometry. Results The proportion of TIGIT+CD4+ T cells was higher in the spleen of S. japonicum?infected mice than in the normal uninfected mice （29.30%±0.70% vs. 3.09%±0.50%； t = 8.834， P < 0.01）. However， no significant difference in the percentages of TIGIT+CD8+T cells between the infection group and normal controls （3.61%±0.26% vs. 3.58%±0.16%； t = 0.108， P > 0.05）， and no significant difference was detected in the percentages of TIGIT+ cells in non?T cells between the infection group and controls （1.86%±0.19% vs.1.37%±0.17%； t = 1.931， P > 0.05）. In addition，the proportion of Ki67 in the TIGIT+ cells was higher than that in the TIGIT? cells （17.03%±0.64% vs. 6.59%±0.37%； t = 14.09， P < 0.01）. The Th2/Th1 ratio was higher in the TIGIT+CD4+ T cells than in the TIGIT?CD4+ T cells （39.28%±3.75% vs. 11.79%±1.64%； t =6.721， P < 0.01）. Conclusion The TIGIT signaling may be involved in the development of Th2 responses in the mice infected with S. japonicum.

Key words: Schistosoma japonicum； TIGIT； Th1/Th2 balance； Flow cytometry