中国血吸虫病防治杂志 ›› 2017, Vol. 29 ›› Issue (6): 683-688.

• 论著 • 上一篇    下一篇

血吸虫对吡喹酮抗药性的研究 ⅩⅦ 日本血吸虫吡喹酮抗性株在小鼠体内的生物学特性

钱科1|2|梁幼生1*|汪伟1|曲国立1|李洪军1|杨振坤1|赵正阳1|邢云天1|戴建荣1   

  1. 1国家卫生和计划生育委员会寄生虫病预防与控制技术重点实验室、江苏省寄生虫与媒介控制技术重点实验室、江苏省血吸虫病防治研究所、江南大学公共卫生研究中心(无锡 214064);2 江西省南昌市疾病预防控制中心
  • 出版日期:2018-01-02 发布日期:2018-01-02
  • 通讯作者: 梁幼生
  • 作者简介:钱科|男|硕士。研究方向:寄生虫病防治及抗血吸虫药物
  • 基金资助:
    国家科技支撑计划(2009BAI78B06);国家自然科学基金(30471516); 江苏省自然科学基金(BK2004024、BK20141105);江苏省科教强卫工程项目;江苏省青年医学人才项目(QNRC2016621);江苏省无锡市卫生局重大科研项目(Z201507)

Studies on resistance of Schistosoma to praziquantel XVII Biological characteristics of praziquantel-resistant isolates of Schistosoma japonicum in mice

QIAN Ke1|2| LIANG You-sheng1*| WANG Wei1| QU Guo-li1| LI Hong-jun1| YANG Zhen-kun1| ZHAO Zheng-yang1| XING Yun-tian1| DAI Jian-rong1   

  1. 1 Key Laboratory of National Health and Family Planning Commission on Technology for Parasitic Disease Prevention and Control| Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology| Jiangsu Institute of Parasitic Diseases|Public Health Research Center of Jiangnan University| Wuxi 214064| China; 2 Nanchang Center for Disease Control and Prevention| Jiangxi Province| China
  • Online:2018-01-02 Published:2018-01-02
  • Contact: LIANG You?sheng

摘要: 目的 实验观察日本血吸虫吡喹酮抗性株在终宿主小鼠体内的生物学特性,探索日本血吸虫吡喹酮抗性株对终宿主的致病力及传播强度。 方法 分别采用日本血吸虫江苏吡喹酮敏感株和抗性株、湖南吡喹酮敏感株和抗性株尾蚴定量感染小鼠,建立小鼠?钉螺?小鼠生活史循环,观察比较各虫株的虫卵开放前期、产卵量及虫卵分布、对终宿主的易感性、虫体生长发育等生物学特性。 结果 日本血吸虫江苏吡喹酮敏感株和抗性株虫卵开放前期分别为36.1 d和36.8 d([t=0.907,P = 0.372]),粪便中虫卵数分别为14.6只/100 mg和21.2只/100 mg([t=2.946,P=0.007]),回收成虫数分别为20.5条/鼠和25.1条/鼠([t=2.128,P=0.042]),组织中虫卵数分别为31 303只/对成虫和38 594只/对成虫([t=2.185,P=0.040])、肝脏虫卵数分别为14 810只/对成虫和19 715只/对成虫([t=2.934,P=0.007]), 肠组织中虫卵数分别为16 493只/对成虫和18 879只/对成虫([t=1.044,P=0.309]);江苏敏感株和抗性株雌雄合抱虫体长度([t=0.328,P=0.744])、雌虫体长([t=0.386,P=0.701])及雄虫体长([t=0.332,P=0.741])差异均无统计学意义。日本血吸虫湖南吡喹酮敏感株和抗性株虫卵开放前期分别为35.5 d和35.6 d([t=0.169,P=0.867]),粪便中虫卵数分别为13.3只/100 mg和18.9只/100 mg([t=3.622,P=0.001]),回收成虫数分别为17.6条/鼠和25.1条/鼠([t=3.153,P=0.004]),组织中虫卵数分别为30 932只/对成虫和53 903只/对成虫(t=3.865,P = 0.001),肝脏虫卵数分别为12 307只/对成虫和26 363只/对成虫([t=4.388,P<0.01]),肠组织中虫卵数分别为18 625个/对成虫和27 541个/对成虫([t=2.679,P=0.012]);湖南敏感株和抗性株雌雄合抱虫体长度([t=0.853,P=0.397])、雌虫体长([t=0.573,P=0.569])及雄虫体长([t=0.742,P=0.461])差异均无统计学意义。 结论 日本血吸虫吡喹酮抗性株产卵量和肝组织虫卵沉积量均明显高于敏感株,提示其对终宿主的致病性更强;抗性株感染鼠粪便中排出虫卵数明显多于敏感株,提示抗性株的传播能量高于敏感株。

关键词: 日本血吸虫;吡喹酮;抗药性;生物学特性;小鼠

Abstract: Objective To investigate the biological characteristics of the praziquantel?resistant isolate of Schistosoma japonicum in mice, so as to explore the pathogenicity to definitive hosts and transmission intensity of the praziquantel?resistant isolate of S. japonicum. Methods Mice were infected with the cercariae released from two praziquantel?resistant isolates and two praziquantel?susceptible isolates of S. japonicum. The mouse?Oncomelania hupensis snail?mouse cycle was established and maintained in the laboratory. The prepatent period of parasite eggs, egg production, egg distribution in mice, parasite susceptibility to mice and egg size were investigated in each parasite isolate. Results The prepatent period of parasite eggs, egg counts in mouse feces, adult worms recovered from each mouse, egg counts in mouse tissues, egg counts in the mouse liver, and egg counts in intestine tissues were 36.1 d and 36.8 d ([t=0.907, P = 0.372]), 14.6/100 mg and 21.2/100 mg ([t=2.946, P= 0.007]), 20.5 and 25.1 worms per mouse ([t=2.128, P = 0.042]), 31 303 and 38 594 per paired adult worm ([t=2.185, P = 0.04]), 14 810 and 19 715 per paired adult worm ([t=2.934, P = 0.007]), and 16 493 and 18 879 per paired adult worm ([t=1.044, P= 0.309]) in the mice infected with Jiangsu praziquantel?susceptible and ?resistant isolates of S. japonicum, respectively, and there were no significant differences between Jiangsu praziquantel?susceptible and ?resistant isolates of S. japonicum in the length of paired adult worms ([t=0.328, P= 0.744]), female adult worms ([t=0.386, P = 0.701]) or male adult worms ([t=0.332, P = 0.741]). The prepatent period of parasite eggs, egg counts in mouse feces, adult worms recovered from each mouse, egg counts in mouse tissues, egg counts in the mouse liver, and egg counts in intestine tissues were 35.5 d and 35.6 d ([t=0.169, P = 0.867]), 13.3/100 mg and 18.9/100 mg ([t=3.622, P = 0.001]), 17.6 and 25.1 worms per mouse ([t=3.153, P = 0.004]), 30 932 and 53 903 per paired adult worm ([t=3.865, P = 0.001]), 12 307 and 26 363 per paired adult worm ([t=4.388, P < 0.01]), and 18 625 and 27 541 per paired adult worm ([t=2.679, P = 0.012]) in the mice infected with Hunan praziquantel?susceptible and ?resistant isolates of S. japonicum, respectively, and there were no significant differences between Hunan praziquantel?susceptible and ?resistant isolates of S. japonicum in the length of paired adult worms ([t=0.853, P = 0.397]), female adult worms ([t=0.573, P = 0.569]) or male adult worms ([t=0.742, P = 0.461]). Conclusions The praziquantel?resistant isolate of S. japonicum has a higher parasite egg production and more eggs deposited in the mouse liver than drug?susceptible isolate, suggesting that the praziquantel?resistant isolate of S. japonicum exhibits a greater pathogenicity to definitive hosts. In addition, more parasite eggs are detected in the feces of mice infected with the praziquantel?resistant isolate of S. japonicum relative to the drug?susceptible isolate, indicating that the praziquantel?resistant isolate of S. japonicum exhibits a greater transmissibility than the drug?susceptible isolate.

Key words: Schistosoma japonicum; Praziquantel; Drug resistance; Biological characteristics; Mouse

中图分类号: