关键词: 日本血吸虫；吡喹酮；抗药性；钉螺；生物学特性

Abstract: Objective To investigate the difference of biological characteristics between the praziquantel?resistant and -susceptible isolates of Schistosoma japonicum in intermediate host Oncomelania hupensis snails. Methods Mice were infected with cercariae of praziquantel?resistant and ?susceptible isolates of S. japonicum， and the parasite eggs were collected 37 days post?infection to hatch miracidium. Then， the snails were infected with the miracidium of each parasite isolate. The snail infection， survival rate of infected snails， prepatent period of cercariae， and the total number of cercariae shed from each infected snail were observed and compared between the praziquantel?resistant and ?susceptible isolates of S. japonicum. Results If each snail was exposed to a single miracidium， there were significant differences between the praziquantel?resistant and ?susceptible Jiangsu isolates in the snail infection （8.99% vs. 19.74%； [χ2]= 3.948， P = 0.047） and the number of cercaria released from a single snail （1 460.2 vs. 1 039.3； t = 2.507， P = 0.02）， and there were significant differences between the praziquantel?susceptible and ?resistant Hunan isolates in the snail infection （10.00% vs. 21.52%； [χ2] = 3.980， P = 0.046） and the number of cercaria releasefrom a single snail （1 319.4 vs. 1 003.5； t = 2.566， P = 0.017）. However， there were no significant differences between the praziquantel?resistant and ?susceptible isolates of S. japonicum in the prepatent period of cercariae and the survival rate of infected snails （P > 0.05）. Conclusion The praziquantel?resistant isolate of S. japonicum has a higher susceptibility to O. hupensis but less cercaria released from each infected snail than the susceptible isolate.

Key words: Schistosoma japonicum； Praziquantel； Drug resistance； Oncomelania hupensis； Biological characteristics