关键词: 刚地弓形虫；抑制蛋白；滴鼻免疫；脾淋巴细胞；免疫应答

Abstract: Objective To observe the splenocytes immune response elicited by different concentrations of recombinant Toxoplasma gondii profilin（rTgPRF） through the nasal route， and determine the optimal dose. Methods Fifty female BALB/c mice were randomly divided into 5 groups. The immunized groups were intranasally administered with 10， 20， 30 μg or 40 μg of rTgPRF that was separately dissolved in 20 μl of phosphate?buffered saline （PBS） on days 0， 14， and 21 respectively， while the control mice were given PBS solution instead. Two weeks after the last immunization， all mice were killed. Under asceptic conditions， the spleens from the immunized mice were dissected， and then the splenocyte proliferative responses in vitro were tested by CCK?8 kit. The levels of IFN?γ， IL?2， IL?4 and IL?10 of splenocyte culture supernatant were detected by ELISA. Results Compared to the control group， the splenocytes from the 30 μg and 40 μg groups exhibited a significantly higher proliferative response to rTgPRF （P < 0.05）， and SI from the 30 μg rTgPRF group was higher than that from the 40 μg group （P <0.05）. The levels of IFN?γ in all the immunized groups （P < 0.05） and IL?2 in the 20， 30 μg and 40 μg groups were significantly stronger than those in the control （P < 0.05）， and the 30 μg group presented the highest concentrations of IFN?γ （P < 0.01）and IL?2 （P < 0.01）. There were no statistical differencesa mong the groups in the levels of IL?4 and IL?10. Conclusions The intranasal immunization with rTgPRF can induce the splenocyteproliferation and Th1?type mediated immunity. The best immunized dose is confirmed as 30 μg.

Key words: Toxoplasma gondii；Profilin； Intranasal immunization；Splenocyte；Immune response