关键词: 东方田鼠, 腹水细胞, 分离培养, 生物学特性

Abstract:

Objective Objective To isolate and culture the spontaneous ascites cells from Microtus fortis under artificial conditions， so as to investigate the molecular mechanism at the cell level. Methods Methods The cells were isolated from spontaneous ascites of M. fortis artificially bred for 90 d，and were cultured and observed under a microscope. The differences of ascites cells among nor? mal，spontaneous ascites and schistosomiasis infected samples of M. fortis were compared. The lesion of tissue was observed si? multaneously. Results Results There were no obvious organ tissue lesions in M. fortis with spontaneous ascites，and the number and types of cells in peritoneal fluid were irregular and significantly changed. With the extension of culture time，the colonies ap? peared and there were a large number of vacuole?like cells in the cultured medium and sequentially presenting proliferation，de? formation，disintegration and the fiber?like changes and could be passaged 3-4 d only. Conclusion Conclusion The cells from M. fortis with spontaneous ascites are similar to its abdominal cavity cells after infection of Schistosoma japonica.

Key words: Microtus fortis, Ascites cell, Isolation and cultivation, Biological characteristic