关键词: 疟疾； 体外药敏实验； WHO微量测试法； 疟原虫乳酸脱氢酶法； 富组蛋白Ⅱ法； SYBR Green Ⅰ法

Abstract: Objective Objective To compare four assay methods of in vitro antimalarial drug efficacy testing，including WHO mi? crotest，Plasmodial lactate dehydrogenase（pLDH），Histidine?rich protein Ⅱ （HRP Ⅱ）and SYBR Green Ⅰ，so as to deter? mine a stable，simple，rapid，and economic method for monitoring the drug sensitivity of malaria parasites and screening new antimalarial drugs. Methods Methods WHO microtest，pLDH，HRP Ⅱ and SYBR Green Ⅰ were applied to test the drug efficacy of chloroquine，piperaquine and amodiaquine against four Plasmodium falciparum reference strains（3D7，FCC，K1 and Dd2）， respectively. The consistency of the 50% inhibitory concentration（IC50 ）values from the four assay methods were analyzed by Friedman tests，Partial correlation analysis，Pearson’ s correlation analysis and Bland?Altman plots. Results Results With the initial parasitemia ranged from 0.5% to 1%，there were no statistically significant differences（P > 0.05）among the IC50 values ob? tained by the four assay methods，which were correlated well（both P < 0.001） . WHO microtest was highly labor?intensive，time? consuming and subjective；although HRP Ⅱ was more sensitive than pLDH and SYBR Green Ⅰ，which was more expensive； SYBR Green Ⅰwas a simple，rapid and economic assay method. Conclusion Conclusion SYBR Green Ⅰ，as a simple and cost?effective assay method，is suitable for high?throughput malaria drug sensitivity monitoring and research of new antimalarial drug screen? ing.

Key words: ］ ］Malaria；In vitro drug efficacy testing；WHO microtest；Plasmodial lactate dehydrogenase（pLDH）；Histidine? rich protein Ⅱ（HRP Ⅱ）； SYBR Green Ⅰ