中国血吸虫病防治杂志

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云南临沧HIV阳性者血液中弓形虫SAG2基因位点分析

贾玉玺1|陈凌娟1|李伟1|聂大平2|罗米3|何建方4|申丽洁5*   

  1. 1大理学院基础医学院寄生虫学教研室(大理 671000);2江西省吉安县人民医院;3重庆医科大学第一临床学院;4 云南省大理市医疗保险管理中心;5 昆明医科大学病原生物学与免疫学系
  • 收稿日期:2014-05-16 出版日期:2014-12-30 发布日期:2014-12-30
  • 通讯作者: 申丽洁
  • 作者简介:贾玉玺|女|硕士研究生。研究方向:分子寄生虫学
  • 基金资助:

    国家自然科学基金(30960330)

Sites analysis of SAG2 gene of Toxoplasma gondii in blood of HIV-positive people of Lincang County in Yunnan Province

JIA Yu-xi1| CHEN Ling-juan1|LI Wei1|NIE Da-ping2|LUO Mi3|HE Jian-fang4|SHEN Li-jie5*   

  1. 1 Department of Parasitology, College of Basic Medical, Dali University, Dali 671000, China|2 Ji'an People's Hospital of Jiangxi Province, China|3 First Clinical College of Chongqing Medical University|China|4 Dali Medical Insurance Management Center, Yunnan Province, China;5 Department of Pathogenic Biology and immunology, Kunming Medical University, China
  • Received:2014-05-16 Online:2014-12-30 Published:2014-12-30
  • Contact: SHEN Li-jie

摘要:

目的 初步了解云南临沧HIV阳性者血液中弓形虫的基因型特征。方法 运用巢式PCR法对弓形虫SAG2基因的两个片段分别进行基因提取、扩增,然后对PCR产物进行回收纯化、酶切及测序,并与弓形虫基因I型标准株进行对比鉴定。结果 从170份HIV阳性者全血样本中分别扩增出35个弓形虫SAG2基因(241、221 bp),从其中扩增阳性的全血样本中各选择4份分别进行酶切,扩增出目的基因片段,进一步从酶切样本中选择2份与弓形虫基因标准株RH株进行对比,酶切SAG2基因显示血液样本基因型为Ⅰ型或Ⅱ型,酶切SAG2基因(221 bp)确定基因型均为Ⅰ型。结论 初步确定云南临沧HIV阳性者弓形虫基因型以Ⅰ型为主。

关键词: 弓形虫;HIV阳性;SAG2基因;基因型;巢式PCR;序列分析

Abstract:

Objective To preliminarily understand the genotype characteristics of Toxoplasma gondii in blood of HIV-positive persons in Lincang City, Yunnan Province. Method Two segments of SAG2 gene of T. gondii from blood samples of the HIV-positive persons were extracted and amplified by using the nested PCR method, and the genotype was identified and compared with the standard strain(Type I) of Toxoplasma gondii. Results Thrity-five SAG2 gene((241 bp) and 35 SAG2 gene((221 bp) of T. gondii were amplified from 170 blood samples of HIV-positive people, and 4 of both were selected and enzyme digested, then 2 aim gene fragments of both were chose and compared with the standard strain(Type I) of Toxoplasma gondii. The digestion of SAG2 gene((241 bp) showed the genotype of the blood samples was Type I or Type II, and the digestion of SAG2 gene((221 bp) determined the genotype was Type I. Conclusion It is preliminarily confirmed that the genotype of Toxoplasma gondii in blood of HIV-positive persons in Lincang City, Yunnan Province is Type I.

Key words: Toxoplasma gondii, HIV-positive, SAG2 gene, Nested PCR, Sequence analysis

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